Professor
Department of Biological Sciences
Towson University
Towson, MD 21252 USA
Office: Smith 347
Phone: 410-704-3120
Fax: 410-704-2405
email: esilldorff@towson.edu
Education:
Post-doc Penn State University School of Medicine
Post-doc University of Maryland School of Medicine
Ph.D. University of Delaware
B.S. University of Delaware
Courses Taught:
BIOL 213/214 - Human Anatomy and Physiology I and II
BIOL 325 - Animal Physiology
BIOL 367 - Endocrinology
BIOL 470 - Advanced Physiology
BIOL 606 - Biochemical Adaptation
Research Interests:
My research interests are in the field of renal (kidney) and cardiovascular physiology; specifically hormonal control of capillary blood flow in the mammalian kidney. Studies examine the contractile characteristics of the kidney microcirculation, specifically the descending vasa recta (a particular capillary network), to determine the potential for regulation of total and regional renal blood flow. Focus is on the effects and interactions of molecules acting in a paracrine or autocrine (local) manner within the cortex (outer region) and medulla (inner region) of the kidney. These blood flow studies provide information about the regulation of the urine concentrating mechanism, blood pressure control, and pathological conditions such as acute ischemic renal failure. Techniques utilized include in vitro microperfusion and videomicroscopy for the measurement of blood vessel contractility as well as fluorescent imaging and photometry for the measurement of cellular signaling events. Fluorescent microscopy allows measurement of changes in intracellular calcium and nitric oxide production, two important mediators of changes in vascular tone. Recently we have added laser-Doppler flowmetry to our tools. This technique allows real-time measurement of regional renal blood flows in the whole animal in response to hormonal stimulation.
Publications:
Lee-Kwon, W., Goo, J.H., Zhang, Z.,
Silldorff, E.P., Pallone, T.L., Vasa recta voltage gated Na+ channel NaV1.3
is regulated by calmodulin, The American Journal of Physiology,
292:F404-F414, 2007
Zhang, Q., Cao, C., Mangano, M.*, Zhang, Z., Silldorff, E.P., Lee-Kwon,
W., Payne, K. and Pallone, T.L., Descending Vasa Recta Endothelium is an
Electrical Syncytium, The American Journal of Physiology,
291:R1688-R1699, 2006
Cao, C., Lee-Kwon, W., Silldorff, E.P., and Pallone, T.L., KATP Channel
Conductance of Descending Vasa Recta Pericytes. American Journal of
Physiology, 289:F1235-F1245, 2005
Hoffman, J.W., Jr.*, Gilbert, T.B., Poston, R.S., and Silldorff, E.P.,
Myocardial Reperfusion Injury: Etiology, Mechanisms, and Therapies. The
Journal of the American Society of ExtraCorporeal Technology, 36:391-411,
2004
Rhinehart, K., Handelsman, C*., Silldorff, E.P., and Pallone, T.L., ANG
II AT-2 receptor modulates AT-1 receptor-mediated descending vasa recta
endothelial calcium signaling. American Journal of Physiology,
284:H779-H789, 2003
Silldorff, E.P., Hilbun, L.* and Pallone, T.L., Angiotensin II
Constriction of Rat Outer Medullary Descending Vasa Recta is Thromboxane
Dependent. Hypertension, 40:541-546, 2002
Silldorff, E.P., and Pallone, T.L., Adenosine signaling in outer
medullary descending vasa recta. American Journal of Physiology
280:R854-R861, 2001
Pallone, T.L. and Silldorff, E.P., Pericyte regulation of renal medullary
blood flow. Experimental Nephrology 9:165-170, 2001
Edwards, A. Silldorff, E.P., and Pallone, T.L., The renal medullary
microcirculation. Frontiers in Bioscience, 5:36-52, 2000
Pallone, T.L., Silldorff, E.P., and Zhang, Z., Inhibition of calcium
signaling in descending vasa recta endothelia by ANG II. Am. J. Physiol.
278:H1248-1255, 2000
Pallone, T.L., Edwards, A., Ma, T., Silldorff, E.P., and Verkman, A.S.,
Requirements of aquaporin-1 for NaCl driven water transport across descending
vasa recta. J. Clin. Invest. 105:215-222, 2000
Graduate Students:
Dana Farrell (Fluorescence microscopy; measurement of intracellular calcium in response to adenosine)
Undergraduate Students:
Layla Hilbun* (Microperfusion and videomicroscopy; role of thromboxane in mediating the response to angiotensin II in vasa recta)
Milana Yusupov (Microperfusion and videomicroscopy)
Sherry Nevab (Microperfusion and videomicroscopy)
Nanna Araiban* (Fluorescence microscopy protocol trials: intracellular calcium imaging)
Nikole Jecen (Fluorescence microscopy: intracellular calcium changes in response to bradykinin and adenosine)
Jodi Rhoderick (Fluorescence microscopy: intracellular calcium changes in response to bradykinin and adenosine)
Corey Handelsman* (Fluorescence microscopy: Inhibition of Angiotensin II Induced Decrease in Endothelial Calcium in Rat Outer Medullary
Descending Vasa Recta
Tara Morgan (Laser Doppler protocol trials)
Hajafatmata Bah (Laser Doppler protocol trials)
Samson Guthua (Laser Doppler protocol trials and test of Bradykinin)
Michael Nassif* (Laser-Doppler: Effects of Bradykinin, Angiotensin II, Angiotensin III, Adenosine, and Endothelin on regional renal blood flows)
Kelvin Bush* (Laser-Doppler: Effects of Bradykinin, Angiotensin II, Angiotensin III, Adenosine, and Endothelin on regional renal blood flows)
* REU students.